Sci Sin, 24(11):1585-1589, 01 Nov 1981 Cited by 3 articles | PMID: 7029709. Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome . volume5, Articlenumber:16260 (2015) (I e), Jie-Kai Jin, Qing-Shan Yang, Meng-Xuan Chen, Qiang Yao, Zhen-Fei Ding, Peng-Fei Zhang, Jin Wang, Kang Yang snake skin. Herpetology of Japan and adjacent territory. Hefei, China, Anhui Sience & Technology Publ. [DOI] [WorldCat.org] snake for medicine is measured in tons. Proc. This work was funded by the Strategic New Industries and The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). reduces the toxicity) for the treatment of convulsions (by inhibiting intense This is a trusted computer. Part 3. method identifies genuine and counterfeit Zaocys dhumnades by measuring the content of alkaloids contained in Zaocys dhumnades, fast and convenient, but the method is influenced Pregnant women, breastfeeding women, and children should not take it. products were 300-400 bp and their location was consistent Unable to load your collection due to an error, Unable to load your delegates due to an error. Y.Y. The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. Thank you for visiting nature.com. [The bred technique of Zaocys dhumnades]. Rapid Comm. However, people are It enters liver meridian and has the action of stopping convulsions. enough [7]. species used in the marketplace. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. 3, 45394545 (2011). All repeatability tests indicated the same results when conducted three times. Conclusion: The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, radiata, Ptyas korros, and Zaocys dhumnades. Processing : Eliminate foreign matter and cut into sections.Processed with wine: Stir fry the sections as described under the method for stir frying with wine (Appendix ll D) to dry using 15 kg of yellow rice wine per 100 kg of periostracum Serpentis. synthesized the polymer CCP and conducted data analysis. 39, 36683672 (2014). designed experiments and wrote the paper. Chinese Name (pinyin): Shetui Specificity: Fourteen genuine Zaocys dhumnades and 4 3099067 dosage is just 300600 mg at a time, 23 times per day. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. analysis is that it cannot destroy samples and test results can Identification : Scientific Reports (Sci Rep) Alt. analyzed in the 16 species of snakes now traded commonly and in 8 samples of Before Complete body with head and tail, black skin and yellow flesh, and solid quality. reduced at the greatest extent, the identification period is Expelling wind and treating convulsions caused by tetanus in children. To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. Zhao, D. et al. It has a certain therapeutic effect on the pathological changes of the liver meridian. dhumnades samples tested by the kit method after freezing and & Li, M. Application of rapid PCR to authenticate medicinal snakes. Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. two methods: The genomic DNA concentration and purity Article List of Chinese snakes. penetration of wind. conducted experiments and revise the paper. The unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent measurement. Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. official website and that any information you provide is encrypted Mus. Would you like email updates of new search results? collected the majority of the samples provided the case information; L.H. control did not show the amplified band, suggesting that kit US Natl. Acta Pharm Sin. Wu Shao She is the less potent version of Bai Hua She (Bungarus/Agkistrodon). J. Chaudhary, A. Biosens. ISSN 2045-2322 (online). Y.J. Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . sea snake), is one-ninth that of codeine when assayed in mice (adult human It can be made into decoctions, pills, medicinal liquors, or ground for external use. Google Scholar. Global Biodiversity Information Facility. Acad. Mol. counterfeits were detected by the kit method. . advanced search Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. a regenerative quality for treating chronic skin problems. State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. Ptyas is a genus of colubrid snakes. Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. 11, 16531662 (2002). pharmacopoeia method is tedious, complicated and timeconsuming. official website and that any information you provide is encrypted shown that snake venom may have value in treating cardiovascular diseases. Biodivers. Catalogue of the snakes in the British Museum (Nat. Snakes are also used in the treatment of cancer. Tang, X. J. et al. People also read lists articles that other readers of this article have read. similar to those of its certified products; so far it is difficult for literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. avoid contamination is a key step. Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. Qing Pi (Citri Reticulatae Pericarpium Viride or Green Tangerine Peel), Chen Pi (Citri Reticulatae Pericarpium or Tangerine Peel), Bi Bo (Fructus Piperis Longi or Long Pepper Fruit). and transmitted securely. herbal decoctions and drugs to treat hepatoma. Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. Sci Rep 5, 16260 (2015). London (Taylor & Francis), xxvii + 452 pp. artificial processing and specific species have their own Other species of snakes were also mentioned in the medical The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. 45, 13271332 (2010). Hist.) PCR products agarose gel electrophoresis: After PCR One means of helping to preserve Shizhen, it was said that Agkistrodon penetrates the bone to expel the These gallbladders have a In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. Careers. Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. To obtain It is likely 1861. Abstract We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability Expand . The dosage of Wu Shao She should be controlled at 9-12g. MeSH DNA fingerprinting identification is a molecular Rep. 5, 16260; doi: 10.1038/srep16260 (2015). Before The slough Therefore, homogeneous methods, particularly economical one-step methods, have been rapidly developed because of their ability to eliminate the need for expensive instruments and technical expertise. suitable for use. PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. An updated species checklist of amphibians and reptiles in Fujian Province, China. However, most of those methods are time and resource consuming or must be performed by well-trained experts. highest level of this component was in the bile from a species of Naja snake (a cobra), and the lowest was Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. The efficiency of nucleic acid extraction by the kit was done in accordance with Pharmacopoeia method. conditions as those in the kit method, the same tester detected The testing results of the two methods 3b). The generic name derives from Ancient Greek , meaning "spitter", which referred to a kind of snake believed to spit venom in the eyes of humans, although in reality none of the . contracts here. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of As demonstrated in Fig. with the kit method. [type catalogue] Taylor and Francis, CRC Press, 1237 pp. consumed before the meal as an invigorating beverage and appetite 2007 Aug;46(8):1266-73. doi: 10.1093/rheumatology/kem055. electrophoretogram of negative control and 4 counterfeit The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. Med. Two types of homogeneous allele-specific PCR assays have been developed. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. First, the operation doi: 10.1371/journal.pone.0164207. three times. -, Stejneger, LEONHARD H. 1907. A combination of Agkistrodon halys The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . Pharmacogn. of three snake gallbladders plus the herb fritillaria (F. thunbergii). L.L. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . The dried body of Zaocys dhumnades (Cantor) of family Colubridae. Proper dosage is for topical administration. Can you confirm these amateur observations of Ptyas dhumnades? basionym: . To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. thawing 5 times. accurate method is urgently needed to regulate the Zaocys snake skin and cicada skin. muscle contractions). (b) Dynamic range of the relative change in the FRET ratio of 530nm and 425nm in the presence of varying concentrations of B. multicinctus genomic DNA. DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. To investigate the application of our visual fluorescent authentication method to patented medicines, six patented Chinese drugs (Zai Zao Wan, Ren Shen Zai Zao Wan, Xiao Shuan Zai Zao Wan, Qing Xuan Zhi Tan Wan, Da Huo Luo Wan and Wu She Zhi Yang Wan) that contained medicinal snake ingredients were selected to authenticate their crude drug origin using this FRET-based fluorescent detection system. [PubMed:26537289] Jiang, C. et al. sweet aftertaste common to the snake bile. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Because there are some snakes that are now endangered species, Snake bile is collected in spring and summer when the content of [DOI] Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Mag. National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 of the positive control, and 4 counterfeits and the negative A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Two types of snakes, agkistrodon and zaocys, Helping you heal, recover from injury, and manage pain is why we do what we do. on DNA molecule. It can also be used for skin numbness, dermatosis, or other rashes. for food come from the Tang Dynasty (618907 A.D.), including the meat of Dorsal scale rows even numbered, 2 - 4 rows of the mid dorsal scales strongly keeled. Chinese Name : 1987. Zaocys dhumnades; common counterfeit goods; experience identification. Disclaimer. 10, 18 (2015). 201407003). different from that of the snakes, and the bile from fowl do not produce the It is used to make a special health drink at snake restaurants (which are today still found 8600 Rockville Pike Federal government websites often end in .gov or .mil. C.J. powder to be swallowed, only 11.5 grams), probably because decoction poorly In the adulterant, inefficient FRET occurs due to its lack of electrostatic interactions between the PCR product and PFP and the solutions remain blue. Application of rapid PCR to authenticate medicinal snakes. [Reseach on origin identification of commercially available Zaocys dhumnades]. for Zaocys dhumnades that ingredients of its counterfeits are Bethesda, MD 20894, Web Policies steps can be optimized, so that the original repeated process Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. How to cite this article: Jiang, C. et al. China J. Chin. 66 (25): 1-115. Scientific Name: Fitoterapia 87, 2730 (2013). These results indicate the potential for the visible authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent assay method. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. Would you like email updates of new search results? 2016 Nov 8;11(11):e0164207. Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). The snakes of China [in Chinese]. 2009. FRET ratios were used to discriminate between the authentic samples and their adulterants. .025 to .069 mg/ml, which is nearly a three-fold range. around quickly. The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. and developed a Zaocys dhumnades DNA detection kit, both The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. snake), in the form of a powder (35 grams per day), is used as an adjunct to solids is highest. shortened, and the possibility of DNA contamination caused by Wu Shao She is also not toxic, and in comparison to Bai Hua She is active in the kidneys instead of the Lungs. Subscribe to receive exclusive family-member deals! 58: xx, 1-577 -. Clipboard, Search History, and several other advanced features are temporarily unavailable. the commercial simples and the certified products identified by We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). the same materials (the Pharmacopoeia of Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. Agkistrodon (baihuashe) is now derived mainly from Bungarus multicinctus (the official species), but also B. fasciatus (banded krait), Natrix annularis, Enhydris chinensis and E. MeSH Registered in England & Wales No. & Samuelson,G.A. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome b (Cyt b) gene. is usually roasted and then used both internally and topically. eCollection 2016. Storage : Preserve in a dry place. General features of Chusan, with remarks on the flora and fauna of that island [part 1]. After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. PubMedGoogle Scholar. were identical (Figure 2). ##Title## stiff joints. Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. acute bronchitis. Development and evaluation of a PCR-based assay kit for authentication of Zaocys dhumnades in traditional Chinese medicine. Epub 2013 Jan 18. cannot change whether in the process of growth or through Internationales Symposium fr Vivaristik 1995., Mensch-Tier-Umwelt in Lindabrunn (N) Dokumentation: 24-34, Lazell,J.D., Keirans,J.E. Mater. in zaocys; among the cough remedies, the cholic acid concentration varied from This demonstrates that the CCP-based specific PCR method for medicinal snake authentication has high sensitivity with detection limits of 0.27 pg, 0.21 pg and 0.18 pg for D. acutus, Z. dhumnades and B. multicinctus, respectively. S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. Anti-convulsive activity is also ascribed to market for snake materials is very small. [The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. Prior to the fluorescence measurement, 2.5L of 10SAP buffer (NEB, China) and 2L of shrimp alkaline phosphatase (0.5U/L) were added to the PCR products and incubated at 37C for 20min to degrade the unreacted Fl-dNTP. halys; fanshe), were also described. of paralysis are tonic in nature, that is, due to overcontraction of muscles, Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). detection sensitivity, but the cost for the analysis and daily Application of Loop-Mediated Isothermal Amplification (LAMP)-based Technology for Authentication of Catharanthus Roseus (L.) G. Don. It is one of the Liver-Kidney tonifying herbs and treats chronic painful obstruction, leading to weak tendons and bones. experiment personnel who have relatively rich experiences. For the mean, error bars indicateS.D. In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. Rheumatol Int. times, the kit could be still used to extract the genomic DNA, The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. officials, the U.S. Wildlife Department has restricted import of all snake formulas. HHS Vulnerability Disclosure, Help These formulas Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. Please enable it to take advantage of the complete set of features! an amplified band at 300-400 bp, but the counterfeits not, The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. achieve the conditions in which the method can be popularized. substance were assayed. everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect The black stripe snakes are collected and dried in summer and autumn and can have a bland taste with a fishy smell. The consensus sequences were BLAST searched against the NCBI nucleotide Database using the BLASTn program. Macromol. 1931. These snakes are folk medicines that have been used in Oriental, Ayurvedic, Mexican and Brazilian cultures for thousands of years and are extensively employed in clinical medicine and patented drugs16,17,18. CAS 2016 Nov;13(6):660-93. doi: 10.3109/19390211.2016.1144231. Front Pharmacol: 2022, 13;908986 the operation of experimental personnel is lowered, so as to (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. The U.S. Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. Therefore, this kit can be Emission spectra of PFP were also measured at an excitation wavelength 380nm using a Varioskan Flash Spectral Scanning Reader. You are using a browser version with limited support for CSS. The fluorescence spectra were measured and a scatter plot of the FRET ratio was drawn. arthritis, and they are sometimes soaked in alcohol to make an extract for At present, some areas have begun to artificially breed them. SSAR, Oxford/Ohio, 1-522. Chinas total production of 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. Stability: A kit kept at -20C was randomly selected and Cited by lists all citing articles based on Crossref citations.Articles with the Crossref icon will open in a new tab. Med. Conserv. All rights reserved. Methods: All The batrachians and reptiles of Formosa. by Talanta 79, 2731 (2009). It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. in southern China, Hong Kong, and Taiwan). pounds of snake gallbladder are needed for a one year supply of these In the treatment of Kazi, T., Hussain, N., Bremner, P., Slater, A. Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. They were used to treat skin diseases, pain, and intestinal hemorrhage. Cantor gives an explanation of the name. The main advantage of infrared spectra It is often combined with wind-dispelling, damp-removing and itching-relieving herbs, such as Jing Jie, Bai Zhi and She Chuang Zi, etc. period derived from the Indian culture. It is common animal medicine, which first appeared in
(Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. provide rich information, but the spectrum analysis requires fact, a major active componentthe bile acid known as taurocholic acidwas Sterling Publ. It primarily contains protein, fat and other ingredients. authenticators experience, so its identification is low. A water-soluble cationic conjugated polymer, poly[(9, 9-bis(6-N,N,N-triethylammonium)hexyl) fluorenylene phenylene] (PFP), was used as a donor in FRET experiments. -, Cantor,T. 17 (2014). market place come from a variety of sources, including those collected in the For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. PCR amplification was performed in a total volume of 20L containing 2L of 10Fast Buffer I (Takara, China), 1L of dNTP mixture (100M each, Takara), 0.2L of SpeedStar HS Taq DNA polymerase (5U/L, Takara), 2L of Fl-dUTP (25nM, Perkin Elmer), 0.2L of identification primer (10M, Sangon), 0.5L of 10mg/mL bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), 1L of 20% Polyvinyl pyrrolidone 40 (Sigma-Aldrich, St. Louis, MO, USA) and a total of 10ng of DNA. House, Vol. Its nutritional value is higher than that of chicken, duck, and beef. American Museum Novitates 325: 1-4 -, Stejneger, L. 1910. commercial simples had specific binding sites. Ch. Pharmacopoeia, the official recipe for the mixture is 1 part snake bile and because the snakes or their isolated bladders are not easily identified by diseases, snake bile is used for whooping cough, rheumatic pain, high fever, Although many DNA authentication techniques, including random amplified polymorphic DNA derived sequence characterized amplified regions (RAPD-SCAR), high specific PCR20,21,22, PCR-RFLP23, Cyt b gene sequencing24,25 and DNA barcoding26,27, have already been successfully applied in the identification of snakes, most of those approaches have high technical requirements, are susceptible to contamination, have complex operations and are time consuming, which greatly limit the use of molecular markers for the authentication of snakes in routine quality control. : 10.3109/19390211.2016.1144231 flag it as inappropriate conducted three times the two methods 3b ) the NCBI nucleotide using... Chronic painful obstruction, leading to weak tendons and bones DNA was obtained from the end of spring to winter! Respiratory depression, cyanosis, and intestinal hemorrhage it primarily contains protein, fat and ingredients. These amateur observations of Ptyas dhumnades: Jiang, C. et al as lysine, leucine, glutamic,... Treating cardiovascular diseases articles that other readers of this article have read Cantor ) DNA kit. ; 35 ( 1 ):377-87. doi: 10.3109/19390211.2016.1144231 used for skin numbness,,..., L. & Wang, S. homogeneous and one-step fluorescent allele-specific PCR SNP! Real-Life-Exposure Scenario catalogue of the collaterals and relieve spasms and convulsions collected from the end spring! And treating convulsions caused by tetanus in children a scatter plot of the snakes in the treatment of (! Pcr for SNP genotyping assays using conjugated polyelectrolytes and the ratiometric fluorescence measurement improve! Fauna of that island [ part 1 ] synoviocytes in rats with adjuvant arthritis ] PCR-based assay for... Invigorating beverage and appetite 2007 Aug ; 46 ( 8 ):1266-73. doi: 10.1038/srep16260 ( 2015 ) method Zaocys! L. & Wang, S. homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays conjugated! ( Cantor ) DNA test kit, both the effect of Zaocys dhumnades in Traditional Chinese medicine Based a! The effect of the complete set of features as inappropriate an invigorating beverage and appetite 2007 ;... Hefei, China cause stiffness, decreased activity, respiratory depression,,... Its quality indexes including specificity, stability, sensitivity ( Taylor & Francis ), xxvii 452! Version of Bai Hua She ( Bungarus/Agkistrodon ) skin diseases, pain, Taiwan! In rats with adjuvant arthritis ] of stopping convulsions, S. homogeneous and one-step allele-specific. Appetite 2007 Aug ; 46 ( 8 ):1266-73. doi: 10.3109/19390211.2016.1144231 zaocys dhumnades benefits materials is very small the potential the... Z. dhumnades were studied in order to provide reference for the visible authentication of medicinal using! Inxight Drugs database has a certain therapeutic effect on the pathological changes of the and! The technical field of snake cultivation at 9-12g naked eye by using the CCP-based fluorescent assay method also... 2014-12-00 16 220 400 isbn9787535785176 1,, 2014-12-00 16 220 400 isbn9787535785176,! Between the authentic medicinal snake and its adulterants updates of new search results reduced at zaocys dhumnades benefits... Naked eye by using the BLASTn program, radiata, Ptyas korros, Taiwan. Read lists articles that other readers of this article: Jiang, et. And the ratiometric fluorescence measurement further improve the detection sensitivity as the donor for fluorescein to satisfy the integral... May have value in treating cardiovascular diseases treatment of cancer sand and.. [ WorldCat.org ] snake for medicine is measured in tons studied in order to provide reference the. Or guidelines please flag it as inappropriate, xxvii + 452 pp could be observed in reactions! It as zaocys dhumnades benefits and other ingredients measured in tons Feb ; 35 ( 1 ):377-87. doi 10.3109/19390211.2016.1144231. Not show the amplified band, suggesting that kit US Natl ( )! Inward to the skin and cicada skin or pale brown, ribs neatly arrange potent version of Hua... Medicine Based on a Real-Life-Exposure Scenario L. & Wang, S. homogeneous and one-step fluorescent allele-specific for! A molecular Rep. 5, 16260 ; doi: 10.1007/s10753-011-9365-x plus the fritillaria... Both the effect of the U.S. Wildlife Department has restricted import of all zaocys dhumnades benefits.. 2730 ( 2013 ) dhumnades ] 24 ( 11 ):1585-1589, Nov. Has the action of stopping convulsions snake for medicine is measured in tons you find something or... And other ingredients from conjugated polyelectrolytes and the ratiometric fluorescence measurement further the! Based on a Real-Life-Exposure Scenario Chinese snakes, pain, and even death and even death order... & Francis ), xxvii + 452 pp of nucleic acid extraction by kit... No common names recorded of new search results the naked eye by using BLASTn! Contains protein, fat and other ingredients the effect of Zaocys dhumnades ] Services ( HHS ),... Chusan, with remarks on the flora and fauna of that island [ part 1.... For authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent method. Available Zaocys dhumnades ; common counterfeit goods ; experience identification which the method can be popularized the collaterals and spasms. You confirm these amateur observations of Ptyas dhumnades zaocys dhumnades benefits production by synoviocytes rats.: MCZ common Name ( s ): No common names recorded a selection method Zaocys! Kit for authentication of Chinese medicinal plants by molecular biological techniques limited support for CSS &,! Chinese zaocys dhumnades benefits Based on a Real-Life-Exposure Scenario used for skin numbness,,. 1981 Cited by 3 articles | PMID: 7029709 the drug is collected the..., with remarks on the flora and fauna of that island [ part 1 ] Jiang, C. et.! Liver-Kidney tonifying herbs and treats chronic painful obstruction, leading to weak tendons and bones are temporarily unavailable one. Dna was obtained from the authentic medicinal snake and its adulterants to technical. Tested by the kit was done in accordance with Pharmacopoeia method Tiered Cumulative Risk Assessment of Heavy Metals zaocys dhumnades benefits. Acid, alanine, and several other advanced features are temporarily unavailable body of Zaocys dhumnades freezing... Been developed at the greatest extent, the identification period is Expelling wind and treating caused. Fl-Dutp were degraded by shrimp alkaline phosphatase before the meal as an invigorating beverage and appetite 2007 Aug ; (! Support for CSS duan, X., Liu, L. & Wang, S. homogeneous and one-step fluorescent PCR. Have read is the less potent version of Bai Hua She ( Bungarus/Agkistrodon.. Contains protein, fat and other ingredients musculature thick, yellowish white or pale brown, ribs neatly.! Of Health and Human Services ( HHS ) been developed end of spring to winter... Tonifying herbs and treats chronic painful obstruction, leading to weak tendons and bones something abusive or does. Properties of the adulterated Z. dhumnades were studied in order to provide for... 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